SAB4200232

Anti-GAD65 antibody , Mouse monoclonal

clone N-GAD65, purified from hybridoma cell culture

• Synonym(s): Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform
• MDL number: MFCD03455043
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: N-GAD65, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~65 kDa
• species reactivity: human, mouse, monkey, rat
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
• isotype: IgG1
• UniProt accession no.: Q05329
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GAD2(2572) ; mouse ... Gad2(14417) ; rat ... Gad2(24380)

Description

General description

Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.

Application

Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.

Biochem/physiol Actions

Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable