SAB4200081

Monoclonal Anti-BOP1 antibody produced in rat

~1.0 mg/mL, clone BOP 6H11, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Block of proliferation 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BOP 6H11, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~100 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using HeLa cell extracts
• UniProt accession no.: Q14137
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BOP1(23246)

Description

General description

Monoclonal Anti- BOP1 (rat IgG1 isotype) is derived from the hybridoma BOP 6H11 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a peptide corresponding to a fragment of human BOP1, coupled to ovalbumin.

Application

Monoclonal Anti-BOP1 antibody produced in rat has been used in several immunochemical techniques including immunoblotting, immunoprecipitation, and immunocytochemistry.

Biochem/physiol Actions

Ribosome biogenesis protein BOP1 is an essential component of A nucleolar complex, PeBoW that is involved in ribosome biogenesis and cell proliferation. Fluctuations in Bop1 protein level were found to affect the assembly and integrity of the whole PeBoW (Pes1, Bop1, and WDR12) complex. Overexpression of Bop1 mRNA is associated with the development of colorectal cancer.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable