RAB0274

Mouse IL-1 β ELISA Kit

for serum, plasma and cell culture supernatant

• Synonym(s): Il-1 beta, Interleukin-1 beta
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; capture ELISA: suitable
• input: sample type plasma; sample type serum; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 5 pg/mL; standard curve range: 2.74-2000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Il1b(16176)

Description

General description

The Mouse IL-1β ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1β in serum, plasma, cell culture supernatants and urine. Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa. IL-1β is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.

Immunogen

Recombinant Mouse IL-1 β

Application

Mouse IL-1 β ELISA Kit has been used to determine the presence of interleukin-1β (IL-1β) in plasma sample. It has also been used to determine the serum concentration levels of IL-1β.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials