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R7397

Sigma-Aldrich

ProtectRNA RNase Inhibitor 500× Concentrate

RNase inhibitor for in situ hybridization assays

Synonym(s):

Ribonuclease inhibitor ProtectRNA

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30 ML
$232.00

$232.00


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30 ML
$232.00

About This Item

UNSPSC Code:
41106300
NACRES:
NA.52

$232.00


In StockDetails


Quality Level

form

liquid

storage temp.

2-8°C

General description

Not recommended in systems where other enzymatic activity is required.

ProtectRNA ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.

Application

ProtectRNA RNase Inhibitor 500× Concentrate has been used in:
  • RNA extraction[1]
  • tissue sectioning and staining for laser capture microscopy[2]
  • the isolation of blood vessels from hippocampi[3]
ProtectRNA RNase Inhibitor 500× Concentrate is an RNase inhibitor.[4][5][6]
A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.

Features and Benefits

  • The 500× concentrate is economical
  • Eliminates the need for special dishwashing and“after washing” cleaning and treatments.

Quantity

2mL treats 1,000ml of solution.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Legal Information

ProtectRNA is a trademark of Sigma-Aldrich Co. LLC

pictograms

FlameCorrosion

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3 - Met. Corr. 1

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

140.0 °F - closed cup

flash_point_c

60 °C - closed cup


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Vomhof-DeKrey E E, et al.
Cells, 11(20), 3283-3283 (2022)
Gene expression analysis of interferon kappa in laser capture microdissected cervical epithelium.
DeCarlo CA
Analytical Biochemistry, 381(1), 59-66 (2008)
Potential neurotoxicity of titanium implants: Prospective, in-vivo and in-vitro study
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Biomaterials, 276, 121039-121039 (2021)
Exposing the Three-Dimensional Biogeography and Metabolic States of Pathogens in Cystic Fibrosis Sputum via Hydrogel Embedding, Clearing, and rRNA Labeling.
DePas WH
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Questions

  1. Hello, I am wondering what the concentration in U/uL of this RNAse inhibitor is. U being units. Best, Charles

    1 answer
    1. Unfortunately, this product is not tested to determine the unit activity. The solution is performance tested with a recommended starting dilution factor of 1:500.

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