R6400

Anti-phospho-Retinoblastoma (pSer^807,811) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• MDL number: MFCD02097167
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: rat, mouse, human
• technique(s): western blot (chemiluminescent): 1:1,000 using human fibroblasts synchronized by serum deprivation
• UniProt accession no.: P06400
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer807/pSer811)
• Gene Information: human ... RB1(5925) ; mouse ... Rb1(19645) ; rat ... Rb1(24708)

Description

General description

RB1 (RB transcriptional corepressor 1) codes for nuclear phosphoprotein pRB (retinoblastoma protein). It is located on human chromosome 13q14. It is a tumor-suppressor gene.

Specificity

Recognizes phosphorylated Rb (Ser⁸⁰⁷/Ser⁸¹¹) and does not react with non-phosphorylated Rb. Does not react with Rb phosphorylated at other sites.

Immunogen

synthetic peptide corresponding to residues around phosphoserine 807/811 of the human retinoblastoma tumor suppressor protein (Rb), conjugated to KLH.

Application

Anti-phospho-Retinoblastoma (pSer807,811) has been used in western blotting.

Biochem/physiol Actions

RB1 (RB transcriptional corepressor 1) plays an important role throughout G1/S phase transition. Absence of RB induces prostate cancer metastasis. It plays an important role in modulating the cell cycle. It also regulates cell stemness and differentiation.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

Preparation Note

Purified using protein A and peptide affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable