PHG0002
TRIS hydrochloride
Synonym(s):
Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
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About This Item
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein/REAXYS Number:
3675235
EC Number:
MDL number:
UNSPSC Code:
12161700
NACRES:
NA.21
Recommended Products
biological source
synthetic
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, microbial, and trace metals; tested
useful pH range
7.0-9.0
pKa (25 °C)
8.1
suitability
suitable for manufacturing use
foreign activity
Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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Application
Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.
Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.
Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.
Other Notes
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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