Skip to Content
MilliporeSigma
All Photos(1)

Documents

PCG2003

Sigma-Aldrich

TruPAGE Precast Gels

4-12%, 10 x 10cm, 12-well

Synonym(s):

Precast Gels for SDS-PAGE, Precast Polyacrylamide Gels

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41105300

description

for use with Life Technologies, XCell SureLock™ XCell I™, XCell II™ Mini-Cell and Bolt™ Mini Gel Tank

shelf life

24 mo. at 2‑8 °C (from the date of manufacture)

packaging

box of 10 ea

concentration

4-12% polyacrylamide

cassette W × L

10 cm × 10 cm

gel thickness

1 mm

well size × volume

12 wells × 35 μL

compatibility

for use with Lonza Ltd PAGEr Minigel Chamber

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Application

TruPAGE Precast Gels 4-12% have been used to separate proteins through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by Western blotting.

Features and Benefits

TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The precast polyacrylamide gels come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.

  • Long Shelf Life - 2 years from the date of manufacture when stored properly.
  • High Performance - Extended full length resoling gels provides improved resolution.
  • Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
  • Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
  • Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
  • Fast - Run times as low as 30 minutes.
  • Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.

TruPAGE Precast Gels Migration Chart
There is no comb to remove, which prevents torn or bent wells. Instead, the cassettes lock well fingers in place. There is also no tape to remove.

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
PAGEr is a trademark of Lonza Group Ltd.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Leucine elicits myotube hypertrophy and enhances maximal contractile force in tissue engineered skeletal muscle in vitro.
Martin N R W, et al.
Journal of Cellular Physiology, 232(10), 2788-2797 (2017)
A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system.
Tang N, et al.
Vaccine, 36(5), 716-722 (2018)
Syed Ahmad et al.
Analytical biochemistry, 574, 46-56 (2019-03-28)
Nicotinic acetylcholine receptors (nAChR) are ion channels which regulate a numerous of neurotransmitters, including acetylcholine, norepinephrine, dopamine, serotonin and glutamate. These receptors are important targets for the study of a plethora of diseases such as Alzheimer's disease, schizophrenia, Parkinson's Disease
Ann Kristin Hansen et al.
BMC musculoskeletal disorders, 18(1), 432-432 (2017-11-08)
The aim was to investigate whether resident chondrocytes in human articular cartilage and in subculture express vitamin D receptor (VDR) and the enzyme that hydroxylates the prohormone 25(OH)D Cartilage from human osteoarthritic knee joints, cultured chondrocytes and cells grown in
John R Christin et al.
Cell reports, 31(10), 107742-107742 (2020-06-11)
Lineage plasticity is important for the development of basal-like breast cancer (BLBC), an aggressive cancer subtype. While BLBC is likely to originate from luminal progenitor cells, it acquires substantial basal cell features and contains a heterogenous collection of cells exhibiting

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service