P9614
Lambda protein phosphatase
liquid, Bacteriophage Lambda, recombinant, expressed in E. coli
Synonym(s):
Lambda-PPase
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20000 UNITS
$430.00
About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
liquid
mol wt
25 kDa
concentration
≥400000 units/mL
shipped in
dry ice
storage temp.
−70°C
General description
Lambda protein phosphatase is a recombinant protein expressed in Escherichia coli. It requires Mn2+ or Ni2+ as activators.[1]
Biochem/physiol Actions
Lambda protein phosphatase has been used to dephosphorylate HeLa cell extracts.[2]
Can be used to release phosphate groups from serine, threonine or tyrosine residues in proteins. Also active on phosphorylated histidine residues.
Unit Definition
One unit will hydrolyze 1 nanomole of p-nitrophenyl phosphate per min at pH 7.5 at 30 deg C.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
Zhuo S
The Journal of Biological Chemistry, 268(24), 17754-17761 (1993)
Proliferating cell nuclear antigen-dependent rapid recruitment of Cdt1 and CRL4Cdt2 at DNA-damaged sites after UV irradiation in HeLa cells.
Ishii T
The Journal of Biological Chemistry, 285(53), 41993-42000 (2010)
P T Cohen et al.
The Biochemical journal, 260(3), 931-934 (1989-06-15)
Infection of Escherichia coli with phage lambda gt10 resulted in the appearance of a protein phosphatase with activity towards 32P-labelled casein. Activity reached a maximum near the point of cell lysis and declined thereafter. The phosphatase was stimulated 30-fold by
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