P7122

Anti-Protein Disulfide Isomerase (DL-11) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Prolyl 4-hydroxylase subunit β (P4HB), Anti-Erp58, Anti-PDI
• MDL number: MFCD01094568
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 57 kDa
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25 μL
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• technique(s): immunoprecipitation (IP): 1-2 μg using RIPA lysate (250-500 μg) of rat NRK cells; indirect immunofluorescence: 2-5 μg/mL using human HeLa cells; western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of mouse NIH3T3 cells
• UniProt accession no.: P07237
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... P4HB(5034) ; mouse ... P4hb(18453) ; rat ... P4hb(25506)

Description

General description

Prolyl 4-hydroxylase subunit β (P4HB) is a redox-regulated thiol-containing protein. The gene encoding this protein is localized on human chromosome 17q25.3.

Protein Disulfide Isomerase is an abundant multifunctional, soluble enzyme (E.C. 5.3.4.1). PDI is expressed in cellular localizations such as the cell surface, cytosol and nucleus. PDI consists of four tandem domains, two of which contain a catalytic site for S-S bond formation. One domain is the main site of noncovalent interaction with other peptides or proteins. PDI has an N-terminal ER signal and C-terminal ER retention KDEL signal sequences.

Immunogen

synthetic peptide corresponding to amino acid residues 498-508 of human protein disulfide isomerase.

Application

Anti-Protein Disulfide Isomerase (DL-11) antibody produced in rabbit has been used in:

• immunofluorescence
• immunoprecipitation
• immunoblotting

Biochem/physiol Actions

Prolyl 4-hydroxylase subunit β (P4HB) acts as a molecular chaperone in the endoplasmic reticulum of cells and also as an oxidoreductase. It associates with steroid hormones and modulates their actions, concentrations and storage. P4HB accelerates the formation of disulphide bonds in proteins and hence aids in their folding.

Protein Disulfide Isomerase serves as a molecular chaperone, that can suppress protein aggregation. PDI has functions as an essential component of two protein complexes: the heterotetramer collagen prolyl 4-hydroxylase and the heterodimer microsomal triglyceride transfer protein. PDI participates in the hydroxylation of prolines in procollagen during collagen synthesis and in the transfer of neutral lipid onto nascent lipoprotein particles. PDI has calcium-dependent transglutaminase activity, which catalyzes the formation of isopeptide bonds.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable