P5400
Pectinesterase from orange peel
lyophilized powder, ≥150 units/mg protein
Synonym(s):
Pectin methylesterase, Pectin pectylhydrolase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
form
lyophilized powder
specific activity
≥150 units/mg protein
composition
Protein, 20-50% biuret
greener alternative product characteristics
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Application
Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene.
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.
Biochem/physiol Actions
Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
Unit Definition
One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.
Physical form
Contains (NH4)2SO4 and sodium chloride
Analysis Note
Protein determined by biuret.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Doungla E Ngouémazong et al.
Carbohydrate research, 348, 69-76 (2012-01-03)
The influence of the degree and pattern of methylesterification (DM and PM, respectively) on the stiffness of Ca(2+)-pectin gels is extensively examined, at various Ca(2+) concentrations. Accordingly, a highly methyl-esterified pectin was selectively de-esterified using NaOH, plant or fungal pectin
Ida Barbara Reca et al.
Plant molecular biology, 79(4-5), 429-442 (2012-05-23)
A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects
Yuri L Dorokhov et al.
PLoS pathogens, 8(4), e1002640-e1002640 (2012-04-13)
Many plants release airborne volatile compounds in response to wounding due to pathogenic assault. These compounds serve as plant defenses and are involved in plant signaling. Here, we study the effects of pectin methylesterase (PME)-generated methanol release from wounded plants
Daniele Del Corpo et al.
Molecular plant pathology, 21(12), 1620-1633 (2020-10-09)
Pectin is synthesized in a highly methylesterified form in the Golgi cisternae and partially de-methylesterified in muro by pectin methylesterases (PMEs). Arabidopsis thaliana produces a local and strong induction of PME activity during the infection of the necrotrophic fungus Botrytis
Mélanie L'Enfant et al.
International journal of biological macromolecules, 132, 1140-1146 (2019-04-13)
The discovery of molecules that can inhibit the action of phytopathogens is essential to find alternative to current pesticides. Pectin methylesterases (PME), enzymes that fine-tune the degree of methylesterification of plant cell wall pectins, play a key role in the
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service