P4591
Pyruvate Oxidase from microorganisms
lyophilized powder, ≥1.5 U/mg
Synonym(s):
Pyruvate:oxygen oxidoreductase (phosphorylating)
Sign Into View Organizational & Contract Pricing
Select a Size
100 UNITS
$321.00
$321.00
Estimated to ship onMarch 29, 2025
All Photos(5)
Select a Size
Change View
100 UNITS
$321.00
About This Item
$321.00
Estimated to ship onMarch 29, 2025
Recommended Products
Application
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.
Biochem/physiol Actions
Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.
Physical properties
Isoelectric point : 4.3
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Unit Definition
One unit will produce 1.0 μmole of H2O2 per min during the conversion of pyruvate and phosphate to acetylphosphate and CO2 at pH 5.7 at 37 °C.
Physical form
Lyophilized powder containing FAD and sugar as stabilizer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe
Das C, et al.
Sensors and Actuators B, Chemical, 238, 633-640 (2017)
Lan-yan Zheng et al.
International journal of oral science, 3(2), 82-89 (2011-04-13)
The objective of this study was to characterize the oxygen dependent regulation of pyruvate oxidase (SpxB) gene expression and protein production in Streptococcus sanguinis (S. sanguinis). SpxB is responsible for the generation of growth-inhibiting amounts of hydrogen peroxide (H2O2) able
Amit Priyadarshi et al.
Biochemical and biophysical research communications, 380(4), 797-801 (2009-04-03)
MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate) synthase belongs to the superfamily of thiamin diphosphate-dependent decarboxylases, which converts isochorismate and 2-oxoglutarate to SHCHC, pyruvate, and carbon dioxide. Here, we report the first crystal structure of apo-MenD from Escherichia coli determined in tetragonal crystal form. The
Lei Chen et al.
Microbiology (Reading, England), 157(Pt 1), 13-20 (2010-09-18)
Hydrogen peroxide (H(2)O(2)), an important substance produced by many members of the genus Streptococcus, plays important roles in virulence and antagonism within a microbial community such as oral biofilms. The spxB gene, which encodes pyruvate oxidase, is involved in H(2)O(2)
Rachel Benisty et al.
Biochimica et biophysica acta, 1801(9), 1098-1104 (2010-07-06)
FabF elongation condensing enzyme is a critical factor in determining the spectrum of products produced by the FASII pathway. Its active site contains a critical cysteine-thiol residue, which is a plausible target for oxidation by H2O2. Streptococcus pneumoniae produces exceptionally
Protocols
Protocol for Assay Procedure for Pyruvate Oxidase Bacterial
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
