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P2545

Millipore

Protein A–Agarose

saline suspension

Synonym(s):

Protein A resin

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About This Item

MDL number:
MFCD00165563
UNSPSC Code:
41106500
NACRES:
NA.56

biological source

Staphylococcus aureus

form

saline suspension

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

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General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Application

Protein A−Agarose has been used in the immunoprecipitation
  • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
  • of prolyl hydroxylase domain 2 N27 cells
  • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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D J McCool et al.
The Biochemical journal, 341 ( Pt 3), 593-600 (1999-07-27)
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to look for possible involvement of the chaperones calreticulin (CRT) and calnexin
Evaluation of a novel methacrylate-based protein a resin for the purification of immunoglobulins and Fc-fusion proteins
McCaw TR, et al.
Biotechnology Progress, 30(5), 1125-1136 (2014)
Hsp90 Co-chaperone p23 contributes to dopaminergic mitochondrial stress via stabilization of PHD2: Implications for Parkinson?s disease
Rane A, et al.
Neurotoxicology, 65, 166-173 (2018)
Madhu Chhanda Mohanty et al.
Developmental and comparative immunology, 26(4), 385-391 (2002-03-13)
Meriones unguiculatus commonly known as gerbils are widely used as animal models for a variety of parasitic infections such as Brugia malayi, Entamoeba histolytica, Giardia duodenalis, Toxoplasma gondi, Helicobacter pylori, Strongyloides stercoralis and Echinococcus multilocularis. Groups of BALB/c mice, gerbils
D J McCool et al.
The Biochemical journal, 302 ( Pt 1), 111-118 (1994-08-15)
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each
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