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MWGF200

Sigma-Aldrich

Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

Synonym(s):

Gel Filtration Protein Ladder

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

form

solid

technique(s)

liquid chromatography (LC): suitable

storage temp.

−20°C

General description

Gel filtration markers kit contains five vials of proteins and 1 vial of blue dextran for calibration.

Application

Gel filtration markers are used in protein chromatography and gel filtration chromatography. Gel filtration markers have been used to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system. Gel filtration markers have also been used to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.

Kit Components Also Available Separately

Product No.
Description
SDS

  • A8531Albumin, bovine serum, 66,000 Da MW 1 mL/vialSDS

  • A8656Alcohol Dehydrogenase, yeast, 150,000 Da MW 1 mL/vialSDS

  • A8781β-Amylase, sweet potato, 200,000 Da MW 1 mL/vialSDS

  • D4772 Blue dextran, 2,000,000 Da MW 1 mL/vialSDS

  • C7025Carbonic Anhydrase, bovine erythrocytes 29,000 Da MW 1 mL/vialSDS

  • C7150Cytochrome c, horse heart, 12,400 Da MW 1 mL/vialSDS

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daisuke Seo et al.
Biochimica et biophysica acta. Bioenergetics, 1861(3), 148140-148140 (2019-12-16)
Among the thioredoxin reductase-type ferredoxin-NAD(P)+ oxidoreductase (FNR) family, FNR from photosynthetic purple non‑sulfur bacterium Rhodopseudomonas palustris (RpFNR) is distinctive because the predicted residue on the re-face of the isoalloxazine ring portion of the FAD prosthetic group is a tyrosine. Here
Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.
Maharjan S, Singh B, Bok JD, et al.
PLoS ONE, 9(5), e96259-e96259 (2014)
Preparation of lectin-vicilin nanoparticle conjugates using the carbodiimide coupling technique.
Ezpeleta, I., et al.
International Journal of Pharmaceutics, 142(2), 227-233 (1996)
Michael Bussmann et al.
The Journal of biological chemistry, 285(38), 29305-29318 (2010-07-21)
The cg1324 gene (rosR) of Corynebacterium glutamicum encodes a MarR-type transcriptional regulator. By a comparative transcriptome analysis with DNA microarrays of a ΔrosR mutant and the wild type and subsequent EMSAs with purified RosR protein, direct target genes of RosR
Fiona McArthur et al.
Journal of bacteriology, 187(15), 5292-5300 (2005-07-21)
The core oligosaccharide component of the lipopolysaccharide can be subdivided into inner and outer core regions. In Escherichia coli, the inner core consists of two 3-deoxy-d-manno-octulosonic acid and three glycero-manno-heptose residues. The HldE protein participates in the biosynthesis of ADP-glycero-manno-heptose

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