The exact number of times the May Grunwald solution can be reused varies depending on factors such as the number of slides stained for each use, temperature, humidity, and exposure to air. Users are expected to evaluate the stain quality for each batch of slides stained and change the solution once the stain quality diminishes. It's important not to return a used working solution back to the stock bottle; instead, used solutions should be stored tightly capped in another bottle, ensuring the cap is always tightly closed to prevent evaporation of methanol and water pickup from the air, which can cause the stain to precipitate out of solution.
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form
solution
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
concentration
0.25 % (w/v) in methanol
technique(s)
microbe id | staining: suitable
application(s)
hematology
histology
storage temp.
room temp
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General description
Application
For use in the differential staining of cellular elements of blood
Preparation Note
Prepared with non-polychromed (non-oxidized) methylene blue and eosin Y.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
target_organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
wgk_germany
WGK 2
flash_point_f
51.8 °F - closed cup
flash_point_c
11 °C - closed cup
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how many times the May-Grunwald stain can be reused before it loses its effectiveness in staining.
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