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MAK559

Sigma-Aldrich

Total Carbohydrate Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Carbohydrate Assay Kit, Carbohydrate Quantification Kit

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About This Item

UNSPSC Code:
41116104
NACRES:
NA.55

usage

sufficient for 100 colorimetric tests

Quality Level

100

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; metabolic disorders

storage temp.

2-8°C

Related Categories

General description

Carbohydrates are the most abundant biomolecules present in all living organisms. Carbohydrates perform various key roles in living organisms-fuel source (glucose), energy storage (glycogen and starch), structural component (cellulose), parts of proteins (glycoproteins, proteoglycans), components of genetic material (ribose and deoxyribose), for survival (trehalose) and more.

The Total Carbohydrate Assay Kit can be used for measuring carbohydrates in a variety of samples, including food and beverage products, solutions, cell culture supernatant and cell lines.

Total carbohydrate concentration is based on the phenol-sulfuric acid method in which polysaccharides are hydrolyzed and then converted to furfural or 5-hydroxymethyllfurfural. These compounds react with the developer to generate a chromogen, which can be detected spectrophotometrically at 490 nm.

Application

Total Carbohydrate Assay Kit has been used in the colorimetric measurement of carbohydrates in food and beverages and biological samples.

Features and Benefits

  • Simple and rapid procedure can be performed in 30 minutes
  • Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file

Suitability

Suitable for measuring total carbohydrates in adherent or suspension cells, animal and plant tissues and food products, fruit juices & other beverages.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
Yue F, et al.
Frontiers in nutrition, 9, 963318-963318 (2022)
Estimating the carbohydrate content of various forms of tobacco by phenol-sulfuric acid method
Jain V M, et al.
Journal of education and health promotion, 6, 90-90 (2017)
Colorimetric measurement of carbohydrates in biological wastewater treatment systems: A critical evaluation
Le C, et al.
Water Research, 94, 280-287 (2016)

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