MAK504
Coenzyme A Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Assay Kit for Coenzyme A Detection
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.84
Recommended Products
Quality Level
detection method
colorimetric
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
shipped in
wet ice
storage temp.
−20°C
General description
Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle, and many others. One of CoA′s most crucial roles is the carrying and transferring of acyl groups.
Features and Benefits
- Linear detection range: 5 - 1000 µM for the colorimetric assay and 3 - 100 µM CoA for the fluorometric assay.
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for Coenzyme A (CoA) determination in a variety of biological samples.
Principle
The Coenzyme A Assay Kit provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the CoA concentration in the sample.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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