MAK475
Creatinine Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.84
Pricing and availability is not currently available.
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Quality Level
detection method
colorimetric
fluorometric
relevant disease(s)
renal disease; neurological disorders; genitourinary disorders
shipped in
wet ice
storage temp.
−20°C
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General description
Creatinine is synthesized in the body from creatine, which is produced from creatine phosphate during muscle contractions. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and subsequent creatin levels in urine are diminished. Creatinine testing is most widely used to assess kidney function.
Features and Benefits
- The linear detection range of creatinine is 4.8 to 500 µM (0.054 - 5.7 mg/dL) for the colorimetric assay and 0.25 to 100 µM (0.0028 - 1.14 mg/dL) for the fluorometric assay.
- Compatible with high-throughput handling systems.
- Convenient. The procedure involves adding a single working reagent and reading after 60 minutes.
Suitability
The kit is suitable for creatinine determination in urine, serum, plasma, and other biological preparations.
Principle
The Creatinine Assay Kit uses a reaction sequence to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the creatinine concentration in the sample. The reaction specifically excludes both endogenous creatine and ammonia.
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Description
Pricing
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
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