1. There is no specific recommended amount of protein per well for testing. It is best to optimize this by testing a range of dilutions to determine the most effective concentration for the specific sample being used.
2. The assay buffer does not contain any inhibitors.
3. Mild sonication can be used instead of a dounce homogenizer, with low amplitude and short pulses, while keeping the samples on ice throughout the sonication process.
MAK356
Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
IDO1 Activity Assay Kit
About This Item
Recommended Products
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
immunological diseases; cancer
General description
Features and Benefits
Suitability
Principle
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
188.6 °F
flash_point_c
87 °C
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1. What is the recommended concentration of protein to use per well?
2. Do the IDO kit buffers contain inhibitors for endogenous enzymes that may metabolize N-formylkynurinine?
3. Will anything in the kit be affected by using sonication instead of a dounce homogenizer?1 answer-
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