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MAK266

Sigma-Aldrich

Triglyceride Quantification Colorimetric/Fluorometric Kit

Triglyceride Quantification Colorimetric/Fluorometric Kit
1 of 3 reviewers received a sample product or took part in a promotion

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

TG Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
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detection method

colorimetric
fluorometric

relevant disease(s)

neurological disorders; cardiovascular diseases

storage temp.

−20°C

General description

The new Triglyceride Quantification Kit, MAK564, is now available! Triglycerides (TG) are the main constituents of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as an energy source.[1][2] TG is broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways.[3] High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.[4]

Suitability

Suitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.

Principle

The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In this assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a colorimetric (570 nm)/fluorometric (lex = 535 nm/lem = 587 nm) product. The kit is sensitive to detect 2 pmole–10 nmole (2–10,000 mM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8K18K06220
8H26K06220
8H11K06220
8F20K06220
8A17K06220

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L Vogel et al.
Journal of dairy science, 103(8), 7431-7450 (2020-06-02)
Rations including high amounts of corn silage are currently very common in dairy production. Diets with corn silage as forage source result in a low supply of essential fatty acids, such as α-linolenic acid, and may lead to low conjugated
David A. Wilson
Clinical Veterinary Advisor: The Horse (2011)
Clinical biochemistry and hematology.
The laboratory rabbit, guinea pig, hamster, and other rodents., 57-116 (2012)
Pingping Luo et al.
Acta pharmaceutica Sinica. B, 11(3), 668-679 (2021-03-30)
Alcoholic liver disease (ALD) causes insulin resistance, lipid metabolism dysfunction, and inflammation. We investigated the protective effects and direct regulating target of S-allylmercaptocysteine (SAMC) from aged garlic on liver cell injury. A chronic ethanol-fed ALD in vivo model (the NIAAA model)
Glyceroneogenesis and the triglyceride/fatty acid cycle.
Reshef L, et al.
The Journal of Biological Chemistry, 278(33), 30413-30416 (2003)
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3 Reviews This action will navigate to reviews.

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Can ethanol or isopropanol (IPA) be used instead of water for liver tissue sample preparation with the Triglyceride Quantification Colorimetric/Fluorometric Kit (MAK266)?

    1 answer

    1. Based on the guidelines, only water is an appropriate solvent for samples in this assay. Ethanol and isopropanol are not suitable solvents for this assay.

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  4. Is liver tissue extracted using RIPA buffer and without NP-40 compatible with the MAK266 Triglyceride Quantification Colorimetric/Fluorometric Kit?

    1 answer

    1. It is recommended to use NP-40 or similar non-ionic detergents instead of RIPA, due to the presence of harsh detergents in RIPA.

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Reviews

1 of 3 reviewers received a sample product or took part in a promotion

Active Filters

  1. Pittsburgh, PA
    • Review 1
    • Votes 8
    1 out of 5 stars.

    Terrible kit!

    The protocol is not well-written. I looked for several literatures who used this kit and found inconsistent data for control samples. Even the technical staffs of Sigma could not satisfy my questions and I strongly discourage other shoppers to use this.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for reaching out and sharing your review with us. We are sorry to hear about your experience with this product and is not what we would expect. Our records show your case was resolved as there were no additional follow up questions. We have provided your feedback to our Product Management team for further review.

  2. 2 Ratings-Only Reviews

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