MAK242
Thrombin Activity Fluorometric Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
Thrombin Enzyme Activity Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
detection method
fluorometric
relevant disease(s)
hematological disorder
storage temp.
−20°C
General description
Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin.
Suitability
Suitable for determining the activity of pure Thrombin and for detecting the activity of Thrombin in plasma.
Principle
Thrombin activity fluorometric assay kit utilizes the ability of thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, 7-amino-4-methylcoumarin (AMC), which can be easily quantified by fluorescence. This assay kit is simple, rapid and can detect thrombin activity as low as 1ng in samples.
Storage Class
10 - Combustible liquids
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The conversion of prothrombin to thrombin I. Characterization of the reaction products formed during the activation of bovine prothrombin.
Owen W G, et al.
The Journal of Biological Chemistry, 249(2), 594-605 (1974)
Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.
Grutter M G, et al.
The Embo Journal, 9(8), 2361-2365 (1990)
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