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MAK012

Sigma-Aldrich

Galactose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Galactose Colorimetric/Fluorometric Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

pediatric diseases; neonatal diseases

storage temp.

−20°C

General description

The new Galactose Assay Kit, MAK502, is now available! Galactose is a simple monosaccharide that serves as an energy source and as an essential component of glycolipids and glycoproteins.[1] Galactose contributes to energy metabolism via its conversion to glucose by the enzymes that constitute the Leloir pathway. Defects in the genes encoding these proteins lead to the metabolic disorder galactosemia.[2]

Application

  • Galactose assay kit has been used in the determination of reactor galactose concentration.[3]
  • It has been used in D-galactose assay using the galactose oxidase enzymatic microreactor.[4]
  • It has also been used in the general synthesis and characterization of O-(2-aminoethyl)-O′-galactosyl polyethylene glycol (H2N-PEG-GAL) derivative.[5]

Suitability

This kit is suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.

Principle

In this assay kit, Galactose is oxidized resulting in a colorimetric (570 nm)/fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the galactose present. This kit has a linear range of detection between 0.2-1.0 nmole galactose for the fluorometric assay and 2-10 nmole galactose for the colorimetric assay.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8H03K06210
8C04K06210
7F04K06210
7A27K06210
6K22K06210

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Rapid and simple preparation of thiol?ene emulsion-templated monoliths and their application as enzymatic microreactors
Josiane P L, et al.
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Joana Silva et al.
Journal of materials chemistry. B, 10(26), 5016-5027 (2022-06-21)
Oxime formation is a convenient one-step method for ligating reducing sugars to surfaces, producing a mixture of closed ring α- and β-anomers along with open-chain (E)- and (Z)-isomers. Here we show that despite existing as a mixture of isomers, N-acetylglucosamine
Galactosylated polyaspartamide copolymers for siRNA targeted delivery to hepatocellular carcinoma cells
Gennara C, et al.
International Journal of Pharmaceutics, 525(2), 397-406 (2017)
A system identification approach for developing model predictive controllers of antibody quality attributes in cell culture processes
Brandon D, et al.
Biotechnol. Progress, 33(6) (2017)
David Papadopoli et al.
Neoplasia (New York, N.Y.), 23(4), 391-399 (2021-03-31)
Notwithstanding that high rates of glucose uptake and glycolysis are common in neoplasia, pharmacological efforts to inhibit glucose utilization for cancer treatment have not been successful. Recent evidence suggests that in addition to classical glucose transporters, sodium-glucose transporters (SGLTs) are
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