M9532
Mannitol Dehydrogenase from Leuconostoc mesenteroides
lyophilized powder, ≥60 units/mg protein
Synonym(s):
polyol dehydrogenases, Mannitol:NAD oxidoreductase
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5 UNITS
$213.75
50 UNITS
$934.80
$213.75
List Price$225.00Save 5%In StockDetails
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5 UNITS
$213.75
50 UNITS
$934.80
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
$213.75
List Price$225.00Save 5%In StockDetails
Recommended Products
form
lyophilized powder
Quality Level
specific activity
≥60 units/mg protein
mol wt
136 kDa
storage temp.
−20°C
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General description
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus.[1] MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.[2]
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. [3] It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol. [4]
The molecular weight of mannitol dehydrogenase is 136 kDa. [5]
Application
Can be used for the production of D-mannitol.
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers. [6]
This preparation is useful in the determination of mannitol in urine.
Biochem/physiol Actions
Unit Definition
One unit will reduce 1.0 μmole of D-fructose per min in the presence of NADH at pH 5.3 at 30°C. One unit of enzyme, as defined above, is equivalent to approximately 1.8 units of enzyme, as described by Lunn et al., one unit will oxidize 1.0 μmole of D-mannitol per min in the presence of NAD at pH 8.6 at 40°C.
Physical form
Lyophilized powder containing buffer salts, potassium phosphate, and dithiothreitol
Preparation Note
Partially purified
Analysis Note
Assayed according to the method of Yamanaka, Meth. Enzymol., 41(B), 138 (1975).
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Polyol metabolism in homopterans at high temperatures: accumulation of mannitol in aphids (Aphididae: Homoptera) and sorbitol in whiteflies (Aleyrodidae: Homoptera)
Hendrix D.L. , Salvucci, M.E.
Comparative Biochemistry and Physiology, 120, 487-494 (1998)
J Aarnikunnas et al.
Applied microbiology and biotechnology, 59(6), 665-671 (2002-09-13)
The N-terminal amino acid sequences of the intact protein and three tryptic peptides from a 41 kDa protein purified from a commercial mannitol dehydrogenase (MDH) enzyme preparation of Leuconostoc mesenteroides ATCC-9135 were determined. Oligonucleotides deduced from these peptide sequences were
Enzymatic production of D-mannitol with the Leuconostoc pseudomesenteroides mannitol dehydrogenase coupled to a coenzyme regeneration system
Parmentier S
Biocatalysis and Biotransformation, 23, 1-7 (2005)
Kevin Blackburn et al.
Rapid communications in mass spectrometry : RCM, 24(7), 1009-1016 (2010-03-10)
Plant cells secrete a wide variety of defense-related proteins into the extracellular space or apoplast in response to pathogen attack. One of these, mannitol dehydrogenase (MTD), is normally a cytoplasmic enzyme whose primary role is the regulation of intracellular levels
P G Lunn et al.
Clinica chimica acta; international journal of clinical chemistry, 183(2), 163-170 (1989-08-15)
A need for a simple method for the determination of mannitol in urine has arisen because of the use of this monosaccharide in intestinal permeability tests. A rapid spectrophotometric assay for mannitol is presented based on the use of the
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