Skip to Content
MilliporeSigma
All Photos(1)

Documents

M9067

Sigma-Aldrich

Anti-MRP1 antibody,Mouse monoclonal

clone QCRL-1, purified from hybridoma cell culture

Synonym(s):

Anti-Multidrug Resistance Associated Protein 1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD01865488
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

QCRL-1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~190 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: 10-20 μg/mL using cultured HeLa cells or HepG2 human hepatocellular carcinoma cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

P33527

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABCC1(4363)

General description

Monoclonal Anti-MRP1 (mouse IgG1 isotype) is derived from the QCRL-1 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from mice immunized with non-denatured membrane preparations of human H69AR small cell lung cancer cell line, which highly expresses MRP1.
Multidrug resistance associated protein 1 (MRP1) is present in subcellular vesicles and is composed of 1531 amino acids. It has a molecular weight of 190kDa. The gene encoding MRP1 is localized in human chromosome 16p13.11.

Specificity

Does not cross-react with the human MDR1 and MDR3, mouse MRP1, and human MRP2, MRP3, MRP4, MRP5, and MRP6 gene products. The antibody may cross-react with canine MRP1.

Immunogen

non-denatured membrane preparations of H69AR human small cell lung cancer cell line, which highly expresses MRP1. The epitope resides within amino acids 918-924 of human MRP1.

Application

Monoclonal Anti-MRP1 antibody produced in mouse has been used in:
  • western blotting
  • immunohistochemistry
  • flow cytometry
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

Multidrug resistance associated protein 1 (MRP1) functions in the metabolism of many drugs. It is involved in transporting different biomolecules and anticancer drugs like prostaglandins, glutathione, alkaloids and anthracyclines. MRP1 offers resistance to chemotherapy to cells in solid tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Characterization of three B-cell lymphoma cell lines from chemotherapy resistant patients with respect to in vitro sensitivity to 21 antitumor agents, ABC-transporter expression and cellular redox status
Bracht K, et al.
Journal of Cancer Research and Clinical Oncology, 133(12), 957-967 (2007)
Shu-Li Shao et al.
Asian Pacific journal of cancer prevention : APJCP, 15(24), 10597-10601 (2015-01-22)
Up-regulation of multidrug resistance-associated protein 1 (MRP1) is regarded as one of the main causes for multidrug resistance (MDR) of tumor cells, leading to failure of chemotherapy-based treatment for a multitude of cancers. However, whether silencing the overexpressed MRP1 is
Yifan Hong et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(11), 3211-3216 (2015-03-05)
Glutathione and multidrug resistance protein (MRP) play an important role on the metabolism of a variety of drugs. Bismuth drugs have been used to treat gastrointestinal disorder and Helicobacter pylori infection for decades without exerting acute toxicity. They were found
Tereza Kunická et al.
PloS one, 9(7), e101740-e101740 (2014-08-01)
ATP-Binding Cassette (ABC) transporters may cause treatment failure by transporting of anticancer drugs outside of the tumor cells. Multidrug resistance-associated protein 1 coded by the ABCC1 gene has recently been suggested as a potential prognostic marker in breast cancer patients.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service