M7070

Anti-MO25 antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CAB39, Anti-CGI-66, Anti-Calcium binding protein 39
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~40 kDa
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~2 mg/mL
• technique(s): western blot: 0.5-1 μg/mL using HEK-293T cells expressing human MO25 and PC12 cell lysates
• UniProt accession no.: Q9Y376
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CAB39(51719) ; mouse ... Cab39(12283) ; rat ... Cab39(301574)

Description

General description

MO25 (mouse protein 25) is also termed calcium binding protein 39, CAB39 and CGI-66 is an Armadillo repeat domain scaffolding protein.

MO25 is a 40 kDa scaffolding protein that interacts with STRADα and LKB1 to regulate tumor suppression. MO25 activates LKB1 and also localizes LKB1 in the cytoplasm .

Specificity

Anti-MO25 antibody is specific for MO25 in humans, rats and mice. In immunoblotting, staining of the MO25 band is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 308-326 located near the C-terminus of human MO25. This sequence is identical in mouse, rat, dog, and bovine MO25.

Application

Anti-MO25 antibody is suitable for use in western blot (0.5-1 μg/mL using HEK-293T cells expressing human MO25 and PC12 cell lysates).

Anti-MO25 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

MO25 (mouse protein 25) binds to Liver kinase B1 (LKB1) and STE20-related kinase adaptor (STRAD) and activates LKB1 and anchors it in the cell cytosol. LKB1 is a key regulator of AMP activated protein kinase (AMPK), which is a master regulator of cellular energy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.