M3694

Anti-α1,2-Mannosidase IA antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-HUMM3, Anti-HUMM9, Anti-MAN9
• MDL number: MFCD07784596
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~73 kDa
• species reactivity: human, rat, mouse
• technique(s): indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells or rat NRK cells; western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of mouse NIH3T3 cells
• UniProt accession no.: P33908
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAN1A1(4121) ; mouse ... Man1a(17155) ; rat ... Man1a1(294410)

Description

General description

α1,2-Mannosidase IA is a type II transmembrane Golgi-resident enzyme that belongs to class I α1,2-Mannosidases (glycosylhydrolase family 47). The highly conserved Class I α1,2-Mannosidases classified into three subgroups according to their enzymatic activities. α1,2-Mannosidase IA is predominantly detected in the juxtanuclear Golgi region. The three subgroups differ in their expression pattern, subcellular localization, and substrate specificity.

Immunogen

synthetic peptide corresponding to amino acids 138-154 of human α1,2-mannosidase IA with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-α1,2-Mannosidase IA antibody produced in rabbit has been used in:

• immunohistochemistry
• immunoblotting
• immunofluorescence

Biochem/physiol Actions

α1,2-Mannosidase IA belongs to the first subgroup out of the three subgroups of class I α1,2-Mannosidases. The first subgroup consists of yeast and human α 1,2-mannosidases of the endoplasmic reticulum that convert Man₉GlcNAc₂ to Man₈GlcNAc₂ isomer B. The second subgroup comprising of mammalian Golgi α1,2-Mannosidases IA, IB, and IC cleave Man₉GlcNAc₂ to Man₅GlcNAc₂ via Man₈GlcNAc₂ isomer A and C. The third subgroup of yeast and mammalian proteins do not show any activity on Man₉GlcNAc₂. Proteins from subgroup 1 and 3 have been implicated in ER quality control and in proteasomal degradation of misfolded glycoproteins. The Golgi mannosidases from the second subgroup may play a role in the ERAD (endoplasmic reticulum-associated degradation) of defective glycoproteins. The three subgroups have been found to pariticipate in the maturation of Asn-linked glycoproteins in the endoplasmic reticulum (ER) and Golgi complex.

Physical form

Solution in 0.01 M phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable