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M2393

Sigma-Aldrich

Magnesium chloride hexahydrate

BioReagent, suitable for cell culture, suitable for insect cell culture

Synonym(s):

MgCl2 hexahydrate, MgCl2.6H2O

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About This Item

Linear Formula:
MgCl2 · 6H2O
CAS Number:
7791-18-6
Molecular Weight:
203.30
EC Number:
232-094-6
MDL number:
MFCD00149781
UNSPSC Code:
12352302
PubChem Substance ID:
24896729
NACRES:
NA.71

product line

BioReagent

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

solubility

H2O: soluble 500 mg/mL, clear, colorless

SMILES string

O.O.O.O.O.O.Cl[Mg]Cl

InChI

1S/2ClH.Mg.6H2O/h2*1H;;6*1H2/q;;+2;;;;;;/p-2

InChI key

DHRRIBDTHFBPNG-UHFFFAOYSA-L

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Application

Magnesium chloride hexahydrate (MgCl2·6H2O) has been used:
  • to prepare electrochemical substrate solution for surface treatment and immunoassay preparation of monoclonal anti-creatine kinase (CK-MB) antibody
  • as a component of casamino acids medium (Hv-Ca) to culture Haloferax volcanii strains
  • to prepare hydrated-salt poly(vinylidenefluoride)-hexafluoropropylene P(VDF-HFP)/MgCl2·6H2O nanocomposite film to study its phase transformation, surface morphology and dielectric property

Biochem/physiol Actions

Magnesium chloride is commonly used as a reagent in chemistry and molecular biology as a source of magnesium ions. Magnesium exhibits several biological roles in enzymology, cell membrane and wall structural integrity, muscle cell physiology, and nucleic acid structure. It functions as a co-factor in various enzymes, like deoxyribonuclease (DNAse), the restriction enzymes EcoRI and EcoRV, and Ribonuclease H. Magnesium stabilizes polymeric nucleic acids including ribozymes and transfer RNA.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Vladimir Riazanski et al.
iScience, 23(11), 101759-101759 (2020-12-01)
The relative contribution of the two phagosomal catabolic processes, oxidative and metabolic, was assessed in the killing of Pseudomonas aeruginosa in phagosomes of alveolar macrophages (AMs) from wild-type (p47-phox +/+ ) or NOX-defective (p47-phox -/- ) mice. Free radical release
Roshali T de Silva et al.
Microbiology (Reading, England), 167(2) (2021-01-19)
Some microbes display pleomorphism, showing variable cell shapes in a single culture, whereas others differentiate to adapt to changed environmental conditions. The pleomorphic archaeon Haloferax volcanii commonly forms discoid-shaped ('plate') cells in culture, but may also be present as rods
Alissa C Rothchild et al.
Bio-protocol, 10(10) (2020-10-01)
Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events
Goli Ardestani et al.
Cell calcium, 87, 102181-102181 (2020-02-26)
Prior to maturation, mouse oocytes are arrested at the germinal vesicle (GV) stage during which they experience constitutive calcium (Ca2+) influx and spontaneous Ca2+ oscillations. The oscillations cease during maturation but Ca2+ influx continues, as the oocytes' internal stores attain
Huiwen Hao et al.
EMBO reports, 21(3), e48385-e48385 (2020-01-28)
Microtubules derived from the Golgi (Golgi MTs) have been implicated to play critical roles in persistent cell migration, but the underlying mechanisms remain elusive, partially due to the lack of direct observation of Golgi MT-dependent vesicular trafficking. Here, using super-resolution
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