L7914
Lipoprotein, low density from human plasma
≥95% (SDS-PAGE), solution
Synonym(s):
β-Lipoprotein, LDL, Low density lipoprotein
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5 MG
$465.00
About This Item
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biological source
human plasma
Quality Level
assay
≥95% (SDS-PAGE)
form
solution
functional group
ester
phospholipid
shipped in
wet ice
Storage temp.
2-8°C
Gene Information
human ... APOB(338) , APOC2(344) , APOE(348)
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Application
Lipoprotein, low density from human plasma has been used:
- as an additive in cholesterol-free RPMI media[1]
- as a component in buffer to perform an assay for measuring lipid peroxidation[2]
- to stimulate hepatic macrophages[3]
- as a plasma protein to determine pazopanib unbound fraction (fu%) by equilibrium dialysis[4]
Biochem/physiol Actions
LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits; receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle resulting dissociation of LDL from its receptor. After loss of the clathrin coat the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake.
Caution
Freezing of lipoprotein solutions may cause structural or compositional changes.
Physical properties
Low density lipoproteins are smaller than VLDL and IDL (26 nm) (MW approximately 3.5 million) and more dense (~1.04). The protein component of LDL is apolipoprotein B100. LDL contains 20-22% protein, 10-15% triglycerides, 20-28% phospholipids, 37-48% cholesteryl esters and 8-10% cholesterol.
Physical form
Solution in 150 mM NaCl and 0.01% EDTA, pH 7.4
Other Notes
View more information on lipoprotein function and lipid transport at www.sigma-aldrich.com/enzymeexplorer
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Isolation and analysis of human plasma lipoproteins accumulating postrandial in an intermediate density fraction (d 1.006--1.019 g-ml).
R Fellin et al.
Clinica chimica acta; international journal of clinical chemistry, 54(3), 325-333 (1974-08-20)
Shu-Ping Hui et al.
Analytical and bioanalytical chemistry, 403(7), 1831-1840 (2012-03-01)
1-Palmitoyl-2-linoleoylphosphatidylcholine monohydroperoxide (PC 16:0/18:2-OOH) and 1-stearoyl-2-linoleoylphosphatidylcholine monohydroperoxide (PC 18:0/18:2-OOH) were measured by liquid chromatography/mass spectrometry (LC/MS) using nonendogenous 1-palmitoyl-2-heptadecenoylphosphatidylcholine monohydroperoxide as an internal standard. The calibration curves for synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, which were obtained by direct injection
Leanne Satchell et al.
Biochemistry, 51(18), 3767-3775 (2012-04-13)
Low-density lipoprotein (LDL) has recently been shown to be oxidized by iron within the lysosomes of macrophages, and this is a novel potential mechanism for LDL oxidation in atherosclerosis. Our aim was to characterize the chemical and physical changes induced
L L Rudel et al.
The Biochemical journal, 139(1), 89-95 (1974-04-01)
1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes
S P Zhao et al.
Clinica chimica acta; international journal of clinical chemistry, 203(2-3), 109-117 (1991-12-16)
After 15 weeks of simvastatin therapy (20 mg/day), low density lipoprotein particle size in sera of 16 patients with type IIb hyperlipoproteinemia increased significantly from 233 +/- 5.0 A to 237 +/- 7.0 A (P less than 0.05), analyzed by
Articles
Lipoproteins package cholesterol for transport in plasma, essential for lipid transport and cellular function in the body.
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