L7395
Lipoxidase from Glycine max (soybean)
Type I-B, lyophilized powder, ≥50,000 units/mg solid
Synonym(s):
15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase
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About This Item
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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type
Type I-B
form
lyophilized powder
specific activity
≥50,000 units/mg solid
mol wt
94 kDa
shipped in
wet ice
storage temp.
−20°C
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General description
Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.
Application
Lipoxidase from Glycine max (soybean) has been used:
- to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
- as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
- as a positive control in lipoxygenase inhibitory activity assay
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Biochem/physiol Actions
Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Unit Definition
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
Physical form
Contains stabilizer and NaCl
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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