This product is tested for solubility at 5 mg/mL in water. Aqueous buffers such as DPBS are expected to be suitable as well. Pease see the link below to review a reference that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685187/
L2637
Lipopolysaccharides from Escherichia coli O55:B5
purified by gel-filtration chromatography
Synonym(s):
LPS
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5 MG
$254.00
10 MG
$482.00
25 MG
$1,010.00
About This Item
Recommended Products
biological source
Escherichia coli (O55:B5)
Quality Level
form
lyophilized powder
purified by
gel-filtration chromatography
impurities
<3% Protein
color
white to faint gray
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
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General description
This product is extracted from E. coli serotype O55:B5 and purified by gel filtration. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/ml and to stimulate equine peritoneal macrophages at 1-100 ng/ml.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Related product
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Related Content
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
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