L2290
Anti-LIM Kinase 1 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-LIMK1
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About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human, mouse, rat
technique(s)
microarray: suitable
western blot: 1:2,000 using a cytosolic fraction of rat brain.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LIMK1(3984)
mouse ... Limk1(16885)
rat ... Limk1(65172)
General description
LIM Domain Kinase 1 (LIMK1) belongs to the LIMK family of serine/threonine protein kinases. LIMK1 shares about 50% sequence identity with LIMK2. LIMK1 contains two N-terminal LIM domains, an internal PDZ (post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (Dlg1), and zonula occludens-1 protein (zo-1)) domain and a C-terminal kinase domain.
Immunogen
Synthetic peptide corresponding to the C-terminus of mouse LIMK1 (amino acids 627-647 or 613-633 of the alternatively spliced forms of LIMK1), conjugated to KLH
This sequence is identical in rat LIMK1 and highly conserved (single amino acid substitution) in human LIMK1. It is not found in the LIMK2 isoform.
This sequence is identical in rat LIMK1 and highly conserved (single amino acid substitution) in human LIMK1. It is not found in the LIMK2 isoform.
Application
Anti-LIM Kinase 1 antibody is suitable for use in microarrays, protein densitometric assays (1:1000 using BPAE cell extracts ) and western blot (1:100, 000 using MDA-MB-231 cell lysates ).
Biochem/physiol Actions
LIMK1 phosphorylates cofilin specifically at Serine 3. This inactivates its F-actin depolymerizing activity and leads to actin reorganization and accumulation of actin filaments. LIMK1 mRNA is highly expressed in the developing nervous system, heart and gut, and in adult brain and spinal cord, suggesting a role for LIMK-1 during neuronal cell differentiation. LIMK1 is localized in the presynaptic nerve ending during the synapse maturation event. LIMK1 also exhibits mitosis-specific activation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Tissue specific characterisation of Lim-kinase 1 expression during mouse embryogenesis
Lindstrom NO, et al.
Gene Expression Patterns, 11(3-4), 221-232 (2011)
Mitosis-specific activation of LIM motif-containing protein kinase and roles of cofilin phosphorylation and dephosphorylation in mitosis
Amano T, et al.
The Journal of biological chemistry, 277(24), 22093-22102 (2002)
J Y Wang et al.
The Journal of comparative neurology, 416(3), 319-334 (1999-12-22)
LIM kinase 1 (LIMK1) is a cytoplasmic protein kinase that is highly expressed in neurons. In transfected cells, LIMK1 binds to the cytoplasmic tail of neuregulins and regulates the breakdown of actin filaments. To identify potential functions of LIMK1 in
Zoe M Goeckeler et al.
The Journal of biological chemistry, 280(38), 33083-33095 (2005-08-02)
This study determined the effects of increased intracellular cAMP and cAMP-dependent protein kinase activation on endothelial cell basal and thrombin-induced isometric tension development. Elevation of cAMP and maximal cAMP-dependent protein kinase activation induced by 10 microm forskolin, 40 microm 3-isobutyl-1-methylxanthine
Patricia Castañeda et al.
Journal of neuroscience research, 93(10), 1476-1491 (2015-05-27)
Chronic stress promotes cognitive impairment and dendritic spine loss in hippocampal neurons. In this animal model of depression, spine loss probably involves a weakening of the interaction between pre- and postsynaptic cell adhesion molecules, such as N-cadherin, followed by disruption
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