KEM0020
T4 DNA Ligase
Ultra-pure enzyme for nucleic acid modifications
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About This Item
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grade
for molecular biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
300,000 U/mg
concentration
120,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Application
Suitable for:
- Restriction cloning
- TA cloning
- Adapter ligation
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Components
Supplied with:KEM0049B (10X T4 DNA Ligase Buffer)
Unit Definition
1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 μL 1X T4 DNA Ligase Buffer following a 30 minute incubation at 23° C.
Physical form
Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.
Other Notes
Source of protein: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.
Unit size: 150,000 U
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Storage Class
10 - Combustible liquids
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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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