I7392
Monoclonal Anti-Interleukin-10 antibody produced in mouse
clone 23738.11, purified immunoglobulin, lyophilized powder
Synonym(s):
Anti-IL-10
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About This Item
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
23738.11, monoclonal
form
lyophilized powder
species reactivity
human
technique(s)
neutralization: 2 μg/mL (ND50)
western blot: 1-2 μg/mL
isotype
IgG2b
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IL10(3586)
General description
Interleukin-10 (IL-10) is a pleotropic cytokine with anti-inflammatory and immunosuppressive functions. It is the key cytokine that regulates the host inflammatory responses to bacterial, fungal and viral pathogens. The production of IL-10 is mediated by signaling of IL-6, IL-12 and TGF-β. IL-10 is secreted by a variety of cells including T cells, B cells, dendritic cells, eosinophils, natural killer cells and mast cells. The effects of IL-10 are mediated by binding to the IL-10R, consisting of α and β subunits. The signaling through IL-10R activates Jak1, Tyk2 protein kinases and JAK/STAT3 pathways. IL-10 is involved in suppression of innate immunity to kill pathogens and inhibits the release of reactive oxygen and nitrogen species from activated macrophages. Regulation of IL-10 production is important in infections, inflammatory responses in autoimmune encephalomyelitis, endotoxic shock and chronic enterocolitis. Anti-interleukin-10 antibody is specific for human IL-10.
Specificity
This antibody will neutralize the biological activity of recombinant human IL-10.
Immunogen
recombinant human IL-10.
Application
Anti-interleukin-10 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody is suitable for neutralization assays at a minimum antibody concentration of 2 μg/ml.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 5% trehalose.
Preparation Note
purified using Protein A or G
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Christian M Hedrich et al.
Immunologic research, 47(1-3), 185-206 (2010-01-21)
IL-10 plays an essential part in controlling inflammation and instructing adaptive immune responses. Consequently, dysregulation of IL-10 is linked with susceptibility to numerous infectious and autoimmune diseases in mouse models and in humans. It has become increasingly clear that appropriate
Joshua C Cyktor et al.
Infection and immunity, 79(8), 2964-2973 (2011-05-18)
The generation of an effective immune response against an infection while also limiting tissue damage requires a delicate balance between pro- and anti-inflammatory responses. Interleukin-10 (IL-10) has potent immunosuppressive effects and is essential for regulation of immune responses. However, the
K W Moore et al.
Annual review of immunology, 19, 683-765 (2001-03-13)
Interleukin-10 (IL-10), first recognized for its ability to inhibit activation and effector function of T cells, monocytes, and macrophages, is a multifunctional cytokine with diverse effects on most hemopoietic cell types. The principal routine function of IL-10 appears to be
Yan Zhang et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 30(3), 113-122 (2010-03-02)
Apoptosis and the rapid clearance of apoptotic cells (ACs) by professional or nonprofessional phagocytes are normal and coordinated processes that ensure controlled cell growth and stress response with nonpathological outcomes. Uptake of ACs by phagocytes is thought to suppress autoimmune
Ivan J Fuss et al.
Journal of immunology (Baltimore, Md. : 1950), 168(2), 900-908 (2002-01-05)
In the present study, we define the relation between TGF-beta and IL-10 in the regulation of the Th1-mediated inflammation occurring in trinitrobenzene sulfonic acid (TNBS)-colitis. In initial studies, we showed that the feeding of trinitrophenol-haptenated colonic protein to SJL/J mice
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