H6166
Interferon α 2B human
IFN-α 2B, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
Synonym(s):
IFN α 2B, IFN Alfa 2b, INF α 2B, INF Alfa 2b, Interferon Alfa 2b
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About This Item
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biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤0.12 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 16 kDa (glycosylated)
packaging
poly bottle of 10 μg (in poly bottle)
manufacturer/tradename
HumanZyme
technique(s)
cell culture | mammalian: suitable
impurities
≤1EU/mg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IFNA2(3440)
General description
The interferon α 2 (IFN-α2) exists in three forms 2a, 2b and 2c. It consists of 165 amino acids and five α helices. IFN-α2B is found structurally stable at pH 7. The recombinant IFN-α is nonglycosylated compared to the natural human IFN-α.
Biochem/physiol Actions
Interferon α 2B (IFN-α 2B) belongs to the type I interferon protein family. It is produced by many cell types in response to viral infection. IFN-α 2B has antiproliferative and antitumor activities, as well as induction of apoptosis and the inflammatory response. It enhances the expression of MHC proteins by increasing the presentation of antigen peptides and activates cytotoxic T-cells.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Preparation Note
HumanKine IFN-α 2B is a glycosylated monomer expressed in HEK 293 cells with an apparent molecular mass of 16 kDa. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note
The specific activity was determined by the dose-dependent proliferation of WISH (heteroploid human amnion cell line) in presence of VSV ( vesicular stomatitis virus).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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