GS90
GST T1-1, Recombinant Human
Synonym(s):
glutathione S-transferase theta 1
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.26
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biological source
human
Quality Level
recombinant
expressed in E. coli
assay
95% (SDS-PAGE)
form
frozen liquid
specific activity
8.8 units/mg protein
mol wt
25 kDa
concentration
1.95 mg/mL
storage temp.
−70°C
Gene Information
human ... GSTT1(2952)
General description
Glutathione S-transferase theta 1 (GSTT1) and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants.GSTT1 is an enzyme that in humans is encoded by the GSTT1 gene. The GSTT1 gene contains five exons and four introns and spans 8092bp on genomic DNA. It is mapped to human chromosome 22q11.23. This gene codes for a 240 amino acids, which is mainly expressed in human liver. Based on their biochemical, immunologic, and structural properties, cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: α, κ, μ, ω, π, σ, θ and ζ.
as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T1-1 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.
Biochem/physiol Actions
Glutathione S-transferase theta 1 (GSTT1) is an enzyme that in humans is encoded by the GSTT1 gene. Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Mutation in the gene might lead to the development of lung, head and neck cancer. The GSTs are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Storage and Stability
The enzyme should be used by the end-user customer within 1 year of receipt.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Association of GSTM1 and GSTT1 gene deletions with risk of head and neck cancer in Pakistan: a case control study
Nosheen, M, et al.
Asian Pacific Journal of Cancer Prevention, 11, 881-885 (2010)
GSTT1 null genotype contributes to lung cancer risk in asian populations: a meta-analysis of 23 studies
Yang, X, et al.
PLoS ONE, 8 (2013)
Dan Wang et al.
Molecular biology reports, 41(11), 7423-7430 (2014-08-05)
Conflicting results in previous case-control studies on the association between Glutathione S-transferase T1 (GSTT1) gene polymorphism and Parkinson's disease (PD) risk have been reported, so we conducted this meta-analysis. We searched and extracted data from 3 Chinese and 3 English
Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defence against oxidative stress
Hayes, J. D, et al.
Free Radical Research, 31, 273-300 (1999)
Alfredo Santovito et al.
Journal of biochemical and molecular toxicology, 29(5), 234-239 (2015-02-04)
Occupational exposure to anaesthetic gases is one of the major hazards to healthcare personnel. We evaluated the cytogenetic effects of chronic exposure to low concentrations of anaesthetic gases in operating theatres. The study included 21 anesthetists and 21 control subjects
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