Skip to Content
MilliporeSigma
All Photos(1)

Documents

GERPN2232

ECL™ Prime Western Blotting System

Cytiva RPN2232

Synonym(s):

Amersham™ ECL™ Prime, Chemiluminescent Western blotting kit, chemiluminescent detection reagent

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2232

color

Detection reagent 1: Colorless Detection reagent 2: Colorless

Related Categories

General description

Amersham™ ECL™ Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.

Amersham™ ECL™ Prime consolidates and builds on the benefits of Amersham™ ECL™ Plus and Amersham™ ECL™ Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

Application

Sensitive quantitative or confirmatory Western Blotting

Features and Benefits

  • High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
  • Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
  • Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham™ Hyperfilm.
  • Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers.
  • Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond-ECL™ (nitrocellulose) membranes.
  • Recommended membrane Hybond-P or Hybon ECL™

Components

The following components are included in the Amersham ECL Prime detection reagent kit.
  • RPN2232 Solution A: Luminol solution, 50 ml Solution B: Peroxide solution, 50 ml sufficient for 1000 cm2 membrane

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Color:
Detection reagent 1: colorless
Detection reagent 2: colorless

Sensitivity: ~1 pg

Legal Information

ECL Prime
This product or portions thereof is manufactured and sold under license from Cyanagen Srl and is subject of US patent 7,855,287, US Patent 7,803,573, and Italian application number TO2010A000580, together with other equivalent granted patents and patent applications in other countries.
Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Rainbow is a trademark of Cytiva

Related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yana van der Weegen et al.
Nature communications, 11(1), 2104-2104 (2020-05-02)
The response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA and UVSSA and the manner in which the core DNA
Cody J Warren et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(23), 11460-11469 (2019-05-23)
Pandemic HIV-1 (group M) emerged following the cross-species transmission of a simian immunodeficiency virus from chimpanzees (SIVcpz) to humans. Primate lentiviruses (HIV/SIV) require the T cell receptor CD4 to enter into target cells. By surveying the sequence and function of

Articles

Water for Protein Electrophoresis & Western Blotting

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Troubleshooting Detection Methods for GST-tagged Proteins

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Protocols

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service