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G7509

Sigma-Aldrich

GABase from Pseudomonas fluorescens

lyophilized powder, Protein ≥30 % by biuret

Synonym(s):

GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase

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10 UNITS
$530.00
25 UNITS
$1,064.70

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10 UNITS
$530.00
25 UNITS
$1,064.70

About This Item

MDL number:
MFCD00131113
UNSPSC Code:
12352204
NACRES:
NA.32

$530.00

In StockDetails

Request a Bulk Order

biological source

Pseudomonas fluorescens

Quality Level

200

assay

≥30  % (biuret)

form

lyophilized powder

specific activity

≥0.5 units/mg protein

composition

Protein, ≥30% biuret

technique(s)

inhibition assay: suitable

storage temp.

−20°C

General description

Research area: Neuroscience


GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens.[1][2]

Application

GABase from Pseudomonas fluorescens has been used for GABase assay to quantify the extracellular and intracellular concentration of GABA (γ-aminobutyric acid).[1][2]

Biochem/physiol Actions

GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.[3]

Unit Definition

One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000412260
0000412260
0000375983
0000359433
0000340581

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Mingu Gordon Park et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 2016-2024 (2021-09-14)
Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification have not been established. Thus, biochemical and drug
Structural and Mechanistic Basis for the Inactivation of Human Ornithine Aminotransferase by (3 S,4 S)-3-Amino-4-fluorocyclopentenecarboxylic Acid
Shen S, et al.
Molecules (Basel), 28(3) (2023)
Kimon-Andreas G Karatzas et al.
Applied and environmental microbiology, 76(11), 3529-3537 (2010-04-20)
It is well established that the glutamate decarboxylase (GAD) system is central to the survival of Listeria monocytogenes at low pH, both in acidic foods and within the mammalian stomach. The accepted model proposes that under acidic conditions extracellular glutamate
Sanjeev Billa et al.
Micromachines, 13(7) (2022-07-28)
Imbalances in levels of glutamate (GLU) and gamma-aminobutyric acid (GABA) and their sub-second signaling dynamics occur in several brain disorders including traumatic brain injury, epilepsy, and Alzheimer's disease. The present work reports on the optimization and in vivo testing of
GABase and glutaminase inhibitory activities of herbal extracts and acylated flavonol monoglycosides isolated from the leaves of Laurus nobilis L
Shimada A, et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 78(9-10), 377-381 (2023)
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Questions

1–4 of 4 Questions  

  1. How do I solubilize Product No. G7509, GABase from Pseudomonas fluorescens?

    1 answer

    1. Sigma recommends to dissolve the product with 75 mM phosphate buffer, pH 7.2, containing 25% (v/v) glycerol to a concentration of 2-5 units per mL. Keep the solution in an ice bath while using and store solution frozen. This solution may be stored frozen and thawed several times with only slight loss of activity.

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  2. What is the shelf life of the Product No.G7509, GABase from Pseudomonas fluorescens?

    1 answer

    1. Sigma recommends retesting the product 3 years after its release date. The retest date will appear on the lot specific Certificate of Analysis for the product.

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  3. Are there any literature references that cite use of the Product No. G7509, GABase from Pseudomonas fluorescens?

    1 answer

    1. The product is cited in the reference Sulaimana, Saba A.J., et al., J of Enz Inhib and Med Chem. 18(4), 297-301 (2003).

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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