The average molecular weight (Mw) of this product is reported as 2,000,000. However, a number average molecular weight (Mn) is not available for this product. Consequently, polydispersity or the Mw/Mn ratio cannot be calculated to determine if this product meets the specified requirements.
FD2000S
Fluorescein isothiocyanate–dextran
average mol wt 2,000,000
Synonym(s):
FITC–Dextran
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100 MG
$102.00
250 MG
$191.00
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$527.00
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$1,900.00
About This Item
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biological source
synthetic (organic)
Quality Level
conjugate
FITC conjugate
form
powder
mol wt
average mol wt 2,000,000
extent of labeling
0.003-0.020 mol FITC per mol glucose
color
yellow to orange
solubility
H2O: soluble 25 mg/mL
storage temp.
2-8°C
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This Item | |||
|---|---|---|---|
| biological source synthetic (organic) | biological source bacterial (Leuconostoc mesenteroides) | biological source synthetic (organic) | biological source synthetic |
| solubility H2O: soluble 25 mg/mL | solubility H2O: 25 mg/mL | solubility H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange | solubility H2O: 25 mg/mL, clear to slightly hazy, yellow-orange |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color yellow to orange | color light yellow to dark orange | color yellow to orange | color faint yellow to orange |
General description
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
Application
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
Fluorescein-dextran, 2000 kDa, was used for in vivo imaging of brain microvasculature in active mice using a minature integrated fluorecent microscope.[1] FITC-dextran, 2000 kDa (FD-2000) is used in cardiovascular, microcirculation, perfusion and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination.[2][3] Fluorescein-dextran, 2000 kDa, may be used for in vivo imaging of brain microvasculature in active mice using a miniature integrated fluorescent microscope. [4] FITC-dextran has been used to study plant cell wall porosity.[5]
Preparation Note
Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.
Other Notes
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Baoqiang Li et al.
PloS one, 14(8), e0221547-e0221547 (2019-08-31)
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Related Content
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| FD2000S-100MG | 04061833033333 |
| FD2000S-5G | 04061833033357 |
| FD2000S-1G | 04061833622384 |
| FD2000S-250MG | 04061833033340 |
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I'm looking for Dextran (Broad MWD, Branched Polysaccharides) D2200K with a WM/MN ratio of approximately 1.0–1.8. Does your product, FD2000S, meet these specifications?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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