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F1631

Sigma-Aldrich

Fast Violet B Salt

Dye content ≥97 %

Synonym(s):

4-Amino-5-methoxy-2-methylbenzanilide diazotated zinc double salt, 4-Benzoylamino-2-methoxy-5-methylbenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 41

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About This Item

Linear Formula:
C15H14N3O2 · 0.5 ZnCl4
CAS Number:
14726-28-4
Molecular Weight:
371.89
Colour Index Number:
37165
EC Number:
238-775-4
MDL number:
MFCD00083330
UNSPSC Code:
12171500
PubChem Substance ID:
24894789

composition

Dye content, ≥97%

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(NC(=O)c2ccccc2)c(C)cc1[N+]#N.COc3cc(NC(=O)c4ccccc4)c(C)cc3[N+]#N

InChI

1S/2C15H13N3O2.4ClH.Zn/c2*1-10-8-13(18-16)14(20-2)9-12(10)17-15(19)11-6-4-3-5-7-11;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2

InChI key

KIWIFIXMWIMRBZ-UHFFFAOYSA-L

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General description

Fast Violet B Salt is a diazonium salt. It is mainly required for cytochemical staining methods, for instance, alkaline phosphatase in leukocytes.

Application

Fast Violet B Salt has been used:
  • for alkaline phosphatase staining in cells
  • for hexosaminidase staining in cells
  • to study lysosomal membrane stability

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Turgeon ML
Clinical Hematology: Theory and Procedures (2005)
Integrated coastal monitoring of a gas processing plant using native and caged mussels.
Brooks S
The Science of the Total Environment, 426, 375-375 (2012)
Beta-glucuronidase and hexosaminidase are marker enzymes for different compartments of the endo-lysosomal system in mussel digestive cells.
Izagirre U
Cell and Tissue Research, 335, 441-441 (2009)
W Cockburn et al.
Analytical biochemistry, 189(1), 95-98 (1990-08-15)
A sensitive, quantitative assay for phosphenolpyruvate carboxylase which utilizes microtiter plates is described. The assay depends upon the production of a colored compound in the reaction between oxaloacetate, the product of the phosphoenolpyruvate reaction, and the dye Fast Violet B.
Jean Rivoal et al.
Analytical biochemistry, 300(1), 94-99 (2001-12-18)
We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance
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