E1131
Exonuclease III from Escherichia coli BE25 /psGR3
buffered aqueous glycerol solution
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About This Item
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grade
for molecular biology
form
buffered aqueous glycerol solution
mol wt
28 kDa
UniProt accession no.
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... xthA(946254)
General description
A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA, therefore reaction conditions must be optimized for specific applications.
Application
Suitable for:
- Production of strand specific probes
- Preparation of single stranded templates for Sanger dideoxy sequencing
- Site directed mutagenesis
Components
Exonuclease III is supplied as a solution in 5 mM potassium phosphate (pH 6.5), 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol.
Unit Definition
One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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Certificates of Analysis (COA)
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Jan Silhan et al.
Nucleic acids research, 40(5), 2065-2075 (2011-11-10)
We have previously demonstrated that the two Exonuclease III (Xth) family members present within the obligate human pathogen Neisseria meningitidis, NApe and NExo, are important for survival under conditions of oxidative stress. Of these, only NApe possesses AP endonuclease activity
Hui Chen et al.
Chemical communications (Cambridge, England), 48(2), 269-271 (2011-11-23)
We describe herein a novel exonuclease III aided amplification method based on single walled carbon nanotube quenching (EASQ) for sensitive and convenient nucleic acid detection, which enabled 80-fold decrease of detection limit for HIV1 DNA assay compared with no target
Lu Peng et al.
Biosensors & bioelectronics, 35(1), 475-478 (2012-04-03)
We have developed a novel DNA assay based on exonuclease III (ExoIII)-induced target recycling and the fluorescence quenching ability of graphene oxide (GO). This assay consists of a linear DNA probe labeled with a fluorophore in the middle. Introduction of
Xu-Hua Zhao et al.
Analytica chimica acta, 727, 67-70 (2012-05-01)
Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection. In the presence of target DNA, the target-probe hybridization forms a double-stranded
L H Guo et al.
Nucleic acids research, 10(6), 2065-2084 (1982-03-25)
We describe improve enzymatic methods for sequencing method for sequencing DNA. They are based on partial digestion of duplex DNA with exonuclease III to produce DNA molecules with 3' ends shortened to varying lengths, followed by repair synthesis to extend
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