DMN70

GenElute^™ Direct mRNA Miniprep Kits

sufficient for 70 purifications

• Synonym(s): GenElute^™ Direct mRNA Miniprep Kit, GenElute^™ mRNA Kit, Gen Elute
• UNSPSC Code: 41105501
• NACRES: NA.52

Properties

• usage: sufficient for 70 purifications
• Quality Level: 200
• storage temp.: 15-25°C

Description

General description

The GenElute^™ Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 10⁷ mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Application

GenElute^™ Direct mRNA Miniprep Kit has been used to isolate mRNA from various tissues and cells.

The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Features and Benefits

• mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
• Poly (A)⁺ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
• Oligo(dT) polystyrene beads require fewer wash steps

Other Notes

Use for isolating mRNA directly from mammalian cells or tissues.

For additional information, please see www.sigma-aldrich.com/mrna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Collection tube 70 ea
• Elution solution 10 mL
• Filtration columns with tubes 70 ea
• Lysis solution 120 mL
• 5 M NaCl 8 mL
• Oligo(dT)-polystyrene beads 2 mL
• Proteinase K 25 mg
Spin columns with tubes 70 ea
40% Glycerol solution 3 mL

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Safety Information

• pictograms: GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H290,H315,H319,H334,H335
• pcodes: P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 8A - Combustible corrosive hazardous materials