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Product Name
ATHOS, 93122325
biological source
Porcine thyroid
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Thyroglobulin, plasminogen activator (u-PA)
receptors
Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Porcine thyroid epithelial
Cell Line Description
The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.
Application
Mechanism of thyroid follicle reconstruction
Culture Medium
Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm2 using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37°C.
Other Notes
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