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Product Name
COLO 839, 93051119
biological source
human blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian B lymphocyte
Cell Line Description
Derived from the peripheral blood of a 43 year old Caucasian male with widespread melanoma. The cells were transformed with defective EBV (B-95-W8).
COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling.
COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 9.3,10
TPOX: 8,11
vWA: 17,19
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 9.3,10
TPOX: 8,11
vWA: 17,19
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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