biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-CD45RA, ovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Sp2/0 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against the p220 component of the sheep CD45 molecule (CD45RA), expressed on all B cells and a sub-population of naive T cells.
Immunogen
Sheep lymphocytes
Application
Useful for distinguishing naive and memory T cells, FACS
Culture Medium
EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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