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product name
Detroit 573, 90112601
biological source
human skin
growth mode
Adherent
karyotype
Aneuploid
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian skin, B/D translocation
Cell Line Description
Detroit 573 was initiated in 1967 from a skin biopsy of a deceased new-born female with multiple congenital anomalies. Approximately 94% of the cell population is 45,XX,t(Bp-;Dq+). The translocated B/D chromosome morphologically resembles a group A-2 chromosome. The cell line is susceptible to polyvirus type 1 and vesicular stomatitis (Indiana Strain) virus.
Application
Karyology studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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