HTK-, 88022409, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human bone
packaging
tube of 5 μg 88022409-DNA-5UG pkg of vial of cells 88022409-1VL
The cells are rendered TK- by selection with 50μg/ml BUdR. HTK- has the same STR profile as 143B (Sigma Catalogue number 91112502), both are TK-; it is likely that they are the same cell line by different names.
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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