RAJI′A′

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85022102, human lymph, Fibroblast-like

• UNSPSC Code: 41106514

Properties

• biological source: human lymph
• packaging: tube of 5 μg 85022102-DNA-5UG; pkg of vial of cells 85022102-1VL
• growth mode: Suspension
• karyotype: Modal no. 47-48
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Burkitt′s lymphoma

Cell Line Description

Clone of RAJI selected for adherence to glass or plastic. Fibroblast like cell line. RAJI ′A′ was developed from the adherent sub-population of RAJI cells. Karyotype analysis demonstrated that RAJI ′A′ contains typical human chromosomes and the modal total chromosome number (47-48) is the same as that of RAJI. RAJI ′A′ is also identical to RAJI with respect to isoenzyme composition, susceptibility to EBV infection and inducibility of latent EBV. Ethnicity: Black.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 17,19

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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