PNV35B
01080924, Reactivity: Anti-Osteoclasts (human foetal), ECV304 and U937 cells
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About This Item
UNSPSC Code:
41106514
ImmunoDon
BALB/c mice
Myeloma
Ag876
Reactivity
Anti-Osteoclasts (human foetal), ECV304 and U937 cells
technique(s)
cell culture | mammalian: suitable
isotype
IgM plus IgG
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Anti-Osteoclastoma Hybridoma
Cell Line Description
135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.
Immunogen
Osteoclastoma tumour
Application
Immunocytochemistry
Culture Medium
Subculture Routine
Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO2, 37°C,
Other Notes
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