PNMR 160D

01080923, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• packaging: tube of 5 μg 01080923-DNA-5UG; pkg of vial of cells 01080923-1VL
• ImmunoDon: BALB/c mice
• Myeloma: SP2
• Reactivity: Anti-Neural Crest (Quail) and human neuroendocrine tissue
• technique(s): cell culture | mammalian: suitable
• isotype: IgM
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Anti-Quail Neural Crest Hybridoma

Cell Line Description

This antibody may provide a valuable tool for studying the differentiation of embryonic precursor cells. The hybridoma supernatant cross reacts with human neuroendocrine cells within appendix, colon and rectum. This mab could provide novel reagents for understanding neural crest development. The antibody is not reactive with somite cultures. The purified antibody is best used at 30 μg/ml for immunocytochemistry. Hybridoma should produce 1-60 μg/ml.

Immunogen

Quail Neural Crest

Application

Immunocytochemistry, diagnostic tools and probes for cellular and macromolecular investigations

Culture Medium

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) + HT Supplement (H0137)

Subculture Routine

Allow hydridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml, 5% CO₂, 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.