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C7339

Sigma-Aldrich

Monoclonal Anti-Cyclin D2 antibody produced in mouse

clone DCS-3, ascites fluid

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About This Item

MDL number:
MFCD00239665
UNSPSC Code:
12352203

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

clone

DCS-3, monoclonal

mol wt

antigen 33-35 kDa (and several lower and higher MW proteins)

contains

15 mM sodium azide

species reactivity

mouse, human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:400 using a cultured human tumor cell line extract

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CCND2(894)
mouse ... Ccnd2(12444)

Specificity

The antibody is useful in antibody-mediated knock-out experiments. The product does not cross-react with other D-type cyclins.

Immunogen

recombinant human cyclin D2 protein.

Certificates of Analysis (COA)

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J Lukas et al.
Oncogene, 10(11), 2125-2134 (1995-06-01)
To explore regulation and function of cyclin D2, a candidate cell cycle-regulatory proto-oncogene, we examined subcellular localisation, cell type- and cell cycle-dependent expression, and requirement of cyclin D2 protein for G1 progression, in a panel of 40 human normal and
Nathalie M Fiaschi-Taesch et al.
Diabetes, 59(8), 1926-1936 (2010-07-30)
Most knowledge on human beta-cell cycle control derives from immunoblots of whole human islets, mixtures of beta-cells and non-beta-cells. We explored the presence, subcellular localization, and function of five early G1/S phase molecules-cyclins D1-3 and cdk 4 and 6-in the
Kikumi Hata et al.
Journal of immunology (Baltimore, Md. : 1950), 173(4), 2453-2461 (2004-08-06)
The engagement of membrane-bound Igs (mIgs) results in growth arrest, accompanied by apoptosis, in the WEHI-231 murine B lymphoma cells, a cell line model representative of primary immature B cells. Inhibitor of differentiation (Id) proteins, members of the helix-loop-helix protein
Ningli Cheng et al.
PloS one, 3(4), e1911-e1911 (2008-04-03)
The effect of Bcl-2 on oncogenesis is complex and expression may either delay or accelerate oncogenesis. The pro-oncogenic activity is attributed to its well characterized anti-apoptotic function while the anti-oncogenic function has been attributed to its inhibition of cellular proliferation.
Amber Yasmeen et al.
Journal of oncology, 2010 (2010-09-24)
In this study, we compared the effects of SKI-606 with Iressa, Src/Abl and EGF-R kinase inhibitors, respectively, on selected parameters in HeLa and SiHa cervical cancer cell lines, which express E6/E7 oncoproteins of high-risk HPV types 18 and 16, respectively.
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