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C5753

Sigma-Aldrich

Chlorophyll a

from spinach

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1 MG
$223.00
5 MG
$881.00
10 MG
$1,206.00

$223.00


Estimated to ship onApril 12, 2025


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1 MG
$223.00
5 MG
$881.00
10 MG
$1,206.00

About This Item

Empirical Formula (Hill Notation):
C55H72MgN4O5
CAS Number:
Molecular Weight:
893.49
Beilstein/REAXYS Number:
3586237
EC Number:
MDL number:
UNSPSC Code:
12171506
PubChem Substance ID:
NACRES:
NA.47

$223.00


Estimated to ship onApril 12, 2025


Request a Bulk Order

biological source

spinach

Quality Level

assay

≥85% (HPLC)

form

powder

impurities

chlorophyll b, essentially free

ε (extinction coefficient)

10.6-11.8 × 104 at 428 nm in diethyl ether (vs. 11.17 × 104, lit.)
8.2-9.1 × 104 at 662 nm in diethyl ether (vs. 8.63 × 104, lit.)

shipped in

dry ice

storage temp.

−20°C

SMILES string

CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67

InChI

1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1

InChI key

ATNHDLDRLWWWCB-AENOIHSZSA-M

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General description

Chlorophyll a is an important light-harvesting, green or blue color[1] pigment present in the photosynthetic membranes of the chloroplasts in plant cells.[2] It functions as an antenna pigment in photosystems I and II.[3]

Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).
Chlorophyll a has been used:
  • to study the variation in carotenoids composition with changing colors and maturation in soybean seeds[4]
  • to examine the use of chlorophyll distribution maps obtained from hyperspectral imaging for the non-destructive diagnosis of Nitrogen deficiency[5]
  • as a standard to determine the concentration of the analyte by fluorescence spectrophotometric technique in the water sample collected from the yellow river[6]
  • as a standard to quantify the amount of chlorophyllous pigments gathered from algae by high performance liquid chromatographic (HPLC) technique[7]
  • in the identification of pheophytin a from environmentally friendly cultivated green peppers[8]
  • to sense different nitric oxide (NO) species[9]

Biochem/physiol Actions

Chlorophyll a binds to the protein molecules CP47 and CP43, forming the inner antenna of the reaction center of Photosystem II. In photosystem I, the binding sites of chlorophyll a includes the polypeptides spread across the membranes.[2] Scarcity in formation of chlorophyll and a low chlorophyll density can lead to deficiency of nitrogen in plants leaves.[5]

Packaging

Sealed ampule.

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Photobiology: The Science of Life and Light (2007)
Nondestructive diagnostics of nitrogen deficiency by cucumber leaf chlorophyll distribution map based on near infrared hyperspectral imaging
Ji-Yong S, et al.
Sci. Hortic., 138, 190-197 (2012)
Lockhart and Wiseman?s Crop Husbandry Including Grassland, 985(2), 101e113-101e113 (2014)
Carotenoid components in soybean seeds varying with seed color and maturation stage
Monma M, et al.
Bioscience, Biotechnology, and Biochemistry, 58(5), 926-930 (1994)
The determination of algal chlorophyllous pigments by high performance liquid chromatography and spectrophotometry
Sartory DP
Water Research, 19(5), 605-610 (1985)

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Is my ampule intact? How can I tell?

    1 answer
    1. If the meniscus "bubble" is not broken, then the ampule is intact and usable. To test the meniscus, use a glass Pasteur pipet and gently touch the bubble. If the glass pops and breaks, then the ampule was sealed. If it goes straight through to the bottom of the ampule with no resistance, then the seal was broken during transit.

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